Enzymes are essential biological catalysts that play a crucial role in many chemical reactions in living organisms. In this practical investigation, we will be exploring the effect of pH on the activity of the enzyme catalase, which is responsible for breaking down hydrogen peroxide into water and oxygen. By carrying out this experiment, we can better understand the role of pH in enzyme activity and how enzymes function in biological systems. Through a step-by-step guide and the use of equipment such as a gas syringe, we can measure the volume of oxygen gas produced and draw conclusions from the data. This practical investigation is a fascinating way to learn about the principles of biological catalysts, chemical reactions, and pH, making it an excellent choice for students interested in biology or chemistry.