In this practical investigation, we explore the world of microscopic observation by examining the unique structures and functions of plant and animal cells. By learning how to use a microscope and observing the cells under low and high magnification, we can gain a better understanding of the characteristics that distinguish plant and animal cells from each other. We provide step-by-step instructions on how to prepare the microscope, how to observe plant and animal cells, and how to draw their structures. We also explain the differences between plant and animal cells, and the purpose of adding iodine solution and methylene blue solution when observing plant and animal cells, respectively. The knowledge gained from this investigation can be applied to many areas of biology, from basic research to medical diagnostics, making it an essential skill for anyone interested in the field.